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cd14 monocyte  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd14 monocyte
    Cd14 Monocyte, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 4679 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+monocytes/CD14+MicroBeads%2C+human/us12673997-882-1-14
    Average 98 stars, based on 4679 article reviews
    cd14 monocyte - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    Modification:

    Article Title: Characterising interleukin-27 (IL-27) responses in human blood derived macrophage cells.
    Article Snippet: Peripheral blood mononuclear cells (PBMC) were isolated using a density gradient centrifugation method [28] incorporating Lymphoprep gradient medium (Fisher Scientific, 17171036) [29,30]. .. CD14+ monocytes were positively selected using human anti-CD14 microbeads (Miltenyi, 130–050-201) [31] and resuspended in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 1 % penicillin-streptomycin. ..

    Magnetic Cell Separation:

    Article Title: Golgi-localised Guanylate-binding protein 5 enhances glycolysis in macrophages
    Article Snippet: .. After PBMCs were obtained, CD14 + Monocytes were extracted using magnetic microbeads (130-050-201, MACS Miltenyi). .. Isolated human monocytes were cultured in complete RPMI medium (10% human AB serum (H4522, Sigma)) (Merck) supplemented with 20 ng/mL human GM-CSF (PeproTech) for macrophage differentiation and incubated for 6 days, adding fresh media on day 3.

    Article Title: Neutrophil-macrophage crosstalk via NETs–IL-17/VEGF/S100A9 axis promotes hepatocellular carcinoma progression
    Article Snippet: Human peripheral blood mononuclear cells (PBMCs) were freshly isolated from healthy donors via Ficoll density gradient centrifugation (P9010, Solarbio, China). .. CD14+ monocytes were further purified using magnetic-activated cell sorting (MACS) with CD14 Microbeads (130-097-052, Miltenyi Biotec, Germany) according to the manufacturer’s instructions. ..

    Isolation:

    Article Title: Single-Cell Dissection Reveals Immune Dysregulation After CD5 or CD7-Directed Chimeric Antigen Receptor T-Cell Therapy.
    Article Snippet: .. Culture and Induction of Human Monocyte-Derived Dendritic Cells: CD14+ monocytes were isolated from PBMCs of HDs using CD14 MicroBeads (130-050-201, Miltenyi, Germany) and cultured in medium containing 100 ng mL−1 hGM-CSF (RP00094, ABclonal, China) and 20 ng mL−1 hIL-4 (RP00995, ABclonal, China). ..

    Cell Culture:

    Article Title: Single-Cell Dissection Reveals Immune Dysregulation After CD5 or CD7-Directed Chimeric Antigen Receptor T-Cell Therapy.
    Article Snippet: .. Culture and Induction of Human Monocyte-Derived Dendritic Cells: CD14+ monocytes were isolated from PBMCs of HDs using CD14 MicroBeads (130-050-201, Miltenyi, Germany) and cultured in medium containing 100 ng mL−1 hGM-CSF (RP00094, ABclonal, China) and 20 ng mL−1 hIL-4 (RP00995, ABclonal, China). ..

    Purification:

    Article Title: Timing of Glucocorticoid Treatment Dictates Glucocorticoid Receptor Actions Modulating the NLRP3‐Inflammasome Activation in Macrophages
    Article Snippet: .. CD14+ monocytes were purified using anti‐human CD14 magnetic beads (Cat# 130‐050‐201, Miltenyi Biotec). .. These purified monocytes were further differentiated into monocyte‐derived macrophages (MoDMs) by culturing in ImmunoCult‐SF Macrophage medium supplemented with human recombinant M‐CSF (50 ng/mL; Peprotech) for 6 days in a CO 2 incubator supplied with 5% CO 2 in a humidified atmosphere.

    Article Title: A novel proteolysis-targeting chimera strategy targeting multiple immune checkpoints containing ITIMs enhances antitumor immunity
    Article Snippet: Purified NK cells were cultured in the presence of Interleukin-2 (IL2, 20 U/mL, AF-200-02; PeproTech, Cranbury, NJ, USA) and interleukin-12 (IL12, 20 ng/mL, 200-12; PeproTech, Cranbury, NJ, USA) to drive their proliferation and survival. .. Monocyte-derived macrophages (MDMs) were generated by culturing purified CD14 + monocytes (130-050-201; Miltenyi Biotec, Bergisch Gladbach, Germany) with recombinant human M-CSF (50 ng/mL; PeproTech) for 7 days. .. Cells were maintained in RPMI-1640 medium or Dulbecco's modified Eagle's medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin (100 U/mL penicillin and 100 μg/mL streptomycin; Cyagen Biosciences, Suzhou, China) at 37 °C in a humidified atmosphere containing 5% CO 2 .

    Article Title: Neutrophil-macrophage crosstalk via NETs–IL-17/VEGF/S100A9 axis promotes hepatocellular carcinoma progression
    Article Snippet: Human peripheral blood mononuclear cells (PBMCs) were freshly isolated from healthy donors via Ficoll density gradient centrifugation (P9010, Solarbio, China). .. CD14+ monocytes were further purified using magnetic-activated cell sorting (MACS) with CD14 Microbeads (130-097-052, Miltenyi Biotec, Germany) according to the manufacturer’s instructions. ..

    Article Title: Characterization of programmed cell death pathways activated in Mycobacterium tuberculosis -infected human macrophages
    Article Snippet: .. CD14+ monocytes were purified using CD14 MicroBeads (Miltenyi Biotech, CA, USA; 130-050-201), with the bead volume reduced to one-third of the manufacturer’s recommended amount. ..

    Article Title: Pharmacological Characterization of GLPG3667, a Tyrosine Kinase 2-Selective Inhibitor, for the Treatment of Inflammatory and Autoimmune Diseases.
    Article Snippet: TNF-α assay in human monocytes PBMCs were isolated from buffy coats using SepMate Isolation Tubes (STEMCELL Technologies, Vancouver, Canada). .. Following red blood cell lysis, CD14+ monocytes were purified by positive selection (130-050-201, Miltenyi Biotec) and resuspended in DMEM F12 medium supplemented with 10% FBS, 100 U penicillin, and 100 μg/mL streptomycin. ..

    Magnetic Beads:

    Article Title: Timing of Glucocorticoid Treatment Dictates Glucocorticoid Receptor Actions Modulating the NLRP3‐Inflammasome Activation in Macrophages
    Article Snippet: .. CD14+ monocytes were purified using anti‐human CD14 magnetic beads (Cat# 130‐050‐201, Miltenyi Biotec). .. These purified monocytes were further differentiated into monocyte‐derived macrophages (MoDMs) by culturing in ImmunoCult‐SF Macrophage medium supplemented with human recombinant M‐CSF (50 ng/mL; Peprotech) for 6 days in a CO 2 incubator supplied with 5% CO 2 in a humidified atmosphere.

    Generated:

    Article Title: A novel proteolysis-targeting chimera strategy targeting multiple immune checkpoints containing ITIMs enhances antitumor immunity
    Article Snippet: Purified NK cells were cultured in the presence of Interleukin-2 (IL2, 20 U/mL, AF-200-02; PeproTech, Cranbury, NJ, USA) and interleukin-12 (IL12, 20 ng/mL, 200-12; PeproTech, Cranbury, NJ, USA) to drive their proliferation and survival. .. Monocyte-derived macrophages (MDMs) were generated by culturing purified CD14 + monocytes (130-050-201; Miltenyi Biotec, Bergisch Gladbach, Germany) with recombinant human M-CSF (50 ng/mL; PeproTech) for 7 days. .. Cells were maintained in RPMI-1640 medium or Dulbecco's modified Eagle's medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin (100 U/mL penicillin and 100 μg/mL streptomycin; Cyagen Biosciences, Suzhou, China) at 37 °C in a humidified atmosphere containing 5% CO 2 .

    Recombinant:

    Article Title: A novel proteolysis-targeting chimera strategy targeting multiple immune checkpoints containing ITIMs enhances antitumor immunity
    Article Snippet: Purified NK cells were cultured in the presence of Interleukin-2 (IL2, 20 U/mL, AF-200-02; PeproTech, Cranbury, NJ, USA) and interleukin-12 (IL12, 20 ng/mL, 200-12; PeproTech, Cranbury, NJ, USA) to drive their proliferation and survival. .. Monocyte-derived macrophages (MDMs) were generated by culturing purified CD14 + monocytes (130-050-201; Miltenyi Biotec, Bergisch Gladbach, Germany) with recombinant human M-CSF (50 ng/mL; PeproTech) for 7 days. .. Cells were maintained in RPMI-1640 medium or Dulbecco's modified Eagle's medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin (100 U/mL penicillin and 100 μg/mL streptomycin; Cyagen Biosciences, Suzhou, China) at 37 °C in a humidified atmosphere containing 5% CO 2 .

    FACS:

    Article Title: Neutrophil-macrophage crosstalk via NETs–IL-17/VEGF/S100A9 axis promotes hepatocellular carcinoma progression
    Article Snippet: Human peripheral blood mononuclear cells (PBMCs) were freshly isolated from healthy donors via Ficoll density gradient centrifugation (P9010, Solarbio, China). .. CD14+ monocytes were further purified using magnetic-activated cell sorting (MACS) with CD14 Microbeads (130-097-052, Miltenyi Biotec, Germany) according to the manufacturer’s instructions. ..

    Red Blood Cell Lysis:

    Article Title: Pharmacological Characterization of GLPG3667, a Tyrosine Kinase 2-Selective Inhibitor, for the Treatment of Inflammatory and Autoimmune Diseases.
    Article Snippet: TNF-α assay in human monocytes PBMCs were isolated from buffy coats using SepMate Isolation Tubes (STEMCELL Technologies, Vancouver, Canada). .. Following red blood cell lysis, CD14+ monocytes were purified by positive selection (130-050-201, Miltenyi Biotec) and resuspended in DMEM F12 medium supplemented with 10% FBS, 100 U penicillin, and 100 μg/mL streptomycin. ..

    Selection:

    Article Title: Pharmacological Characterization of GLPG3667, a Tyrosine Kinase 2-Selective Inhibitor, for the Treatment of Inflammatory and Autoimmune Diseases.
    Article Snippet: TNF-α assay in human monocytes PBMCs were isolated from buffy coats using SepMate Isolation Tubes (STEMCELL Technologies, Vancouver, Canada). .. Following red blood cell lysis, CD14+ monocytes were purified by positive selection (130-050-201, Miltenyi Biotec) and resuspended in DMEM F12 medium supplemented with 10% FBS, 100 U penicillin, and 100 μg/mL streptomycin. ..



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    Image Search Results


    PBMCs were stained for an 11-color panel across 55 samples (NCAVD, n = 20; CAVD, n = 15; volunteers, n = 20). Each panel included lineage markers CD20, CD3, CD4, CD8, and CD25 to exclude B and T lymphocytes, and monocyte markers CD14 and CD16 to identify monocyte subsets. Additional markers CD86, CD163, and CD206 were used to characterize monocyte polarization toward unpolarized, pro-inflammatory, or immunomodulatory macrophage phenotypes, respectively. The gating strategy for identifying these populations is shown for each group.

    Journal: bioRxiv

    Article Title: Patient-Derived Circulating Monocytes Promote Calcific Aortic Valve Disease Progression

    doi: 10.64898/2026.04.30.721898

    Figure Lengend Snippet: PBMCs were stained for an 11-color panel across 55 samples (NCAVD, n = 20; CAVD, n = 15; volunteers, n = 20). Each panel included lineage markers CD20, CD3, CD4, CD8, and CD25 to exclude B and T lymphocytes, and monocyte markers CD14 and CD16 to identify monocyte subsets. Additional markers CD86, CD163, and CD206 were used to characterize monocyte polarization toward unpolarized, pro-inflammatory, or immunomodulatory macrophage phenotypes, respectively. The gating strategy for identifying these populations is shown for each group.

    Article Snippet: CD14 + monocyte RNA samples extracted from volunteers (Vol, n=5), NCAVD (n=5), and CAVD (n=5) patients, with at least 400 ng of total RNA, with a concentration >20 ng/ml and a RIN ratio >8, were sent to Novogene Company Ltd (Cambridge, UK) for library preparation, mRNA sequencing, and bioinformatic analysis.

    Techniques: Staining

    IL4 and CCL3 mRNA expression were quantified by RT-qPCR (A) and normalized to the housekeeping gene B2M . Sample sizes were as follows: for IL4 , n = 8 per group; for CCL3 , n = 8 in the NCAVD and healthy volunteers (Vol) groups and n = 6 for CAVD group. (B) IL-4 and CCL3 CD14 + monocytes secretion were quantified by multiplex immunoassays. Sample sizes were as follows: for IL-4, n = 6 per group; for CCL3, n = 6 in the healthy volunteers (Vol) group and n = 5 for NCAVD and CAVD groups. Asterisks (*) indicate statistically significant differences compared to the healthy volunteer condition (blue) as determined by One-Way ANOVA followed by Sidak’s post-hoc test. Outliers were removed. p-values are indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: bioRxiv

    Article Title: Patient-Derived Circulating Monocytes Promote Calcific Aortic Valve Disease Progression

    doi: 10.64898/2026.04.30.721898

    Figure Lengend Snippet: IL4 and CCL3 mRNA expression were quantified by RT-qPCR (A) and normalized to the housekeeping gene B2M . Sample sizes were as follows: for IL4 , n = 8 per group; for CCL3 , n = 8 in the NCAVD and healthy volunteers (Vol) groups and n = 6 for CAVD group. (B) IL-4 and CCL3 CD14 + monocytes secretion were quantified by multiplex immunoassays. Sample sizes were as follows: for IL-4, n = 6 per group; for CCL3, n = 6 in the healthy volunteers (Vol) group and n = 5 for NCAVD and CAVD groups. Asterisks (*) indicate statistically significant differences compared to the healthy volunteer condition (blue) as determined by One-Way ANOVA followed by Sidak’s post-hoc test. Outliers were removed. p-values are indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: CD14 + monocyte RNA samples extracted from volunteers (Vol, n=5), NCAVD (n=5), and CAVD (n=5) patients, with at least 400 ng of total RNA, with a concentration >20 ng/ml and a RIN ratio >8, were sent to Novogene Company Ltd (Cambridge, UK) for library preparation, mRNA sequencing, and bioinformatic analysis.

    Techniques: Expressing, Quantitative RT-PCR, Multiplex Assay

    The secretome of CAVD CD14 + monocytes is depleted in cytokines involved in T lymphocyte recruitment (CXCL9, CCL21) and Th2 response promotion (CCL17, CCL22). Data are normalized to the housekeeping gene B2M. Asterisks (*) indicate statistically significant differences compared to healthy volunteer samples (blue), determined by one-way ANOVA followed by Sidak’s post-hoc test. Outliers were removed. Statistical significance is denoted as *p < 0.05 and ***p < 0.001.

    Journal: bioRxiv

    Article Title: Patient-Derived Circulating Monocytes Promote Calcific Aortic Valve Disease Progression

    doi: 10.64898/2026.04.30.721898

    Figure Lengend Snippet: The secretome of CAVD CD14 + monocytes is depleted in cytokines involved in T lymphocyte recruitment (CXCL9, CCL21) and Th2 response promotion (CCL17, CCL22). Data are normalized to the housekeeping gene B2M. Asterisks (*) indicate statistically significant differences compared to healthy volunteer samples (blue), determined by one-way ANOVA followed by Sidak’s post-hoc test. Outliers were removed. Statistical significance is denoted as *p < 0.05 and ***p < 0.001.

    Article Snippet: CD14 + monocyte RNA samples extracted from volunteers (Vol, n=5), NCAVD (n=5), and CAVD (n=5) patients, with at least 400 ng of total RNA, with a concentration >20 ng/ml and a RIN ratio >8, were sent to Novogene Company Ltd (Cambridge, UK) for library preparation, mRNA sequencing, and bioinformatic analysis.

    Techniques: